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Proteomic Analysis of the Chlorophyta Dunaliella New Strain AL-1 Revealed Global Changes of Metabolism during High Carotenoid Production ArchiMer
Ben Amor, Faten; Elleuch, Fatma; Ben Hlima, Hajer; Garnier, Matthieu; Saint-jean, Bruno; Barkallah, Mohamed; Pichon, Chantal; Abdelkafi, Slim; Fendri, Imen.
The green microalgae Dunaliella genus is known for the production of high added value molecules. In this study, strain AL-1 was isolated from the Sebkha of Sidi El Hani (Sousse, Tunisia). This isolate was identified both morphologically and genetically via 18S rRNA gene sequence as a member of the genus Dunaliella. Strain AL-1 was found to be closely related to Dunaliella salina, Dunaliella quartolecta and Dunaliella polymorpha with more than 97% similarity. Response surface methodology was used to maximize carotenoid production by strain AL-1 by optimizing its growth conditions. The highest carotenoid content was obtained at salinity: 51, light intensity: 189.89 μmol photons·m−2·s−1, and nitrogen: 60 mg·L−1. Proteomic profiling, using two-dimensional gel...
Tipo: Text Palavras-chave: Proteome; Dunaliella; 18S rRNA gene; Carotenoids; Bidimensional gel electrophoresis; Mass spectrometry.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00402/51398/51973.pdf
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R. nukuhivensis acts by reinforcing skin barrier function, boosting skin immunity and by inhibiting IL-22 induced keratinocyte hyperproliferation ArchiMer
Abdallah, Florence; Lecellier, Gael; Raharivelomanana, Phila; Pichon, Chantal.
Rauvolfia nukuhivensis is a well-known plant used for its wide range of beneficial effects in Marquesas islands. It is made up of diverse indole alkaloids and is used as traditional medicine for skin application. The actual mechanism behind the virtue of this plant is still unknown. Hence, in this study we aimed at deciphering the impact of R. nukuhivensis on skin immune system in context of (1) homeostasis, (2) pathogen infection and (3) inflammation. Here we show that R. nukuhivensis enhances cellular metabolic activity and wound healing without inducing cellular stress or disturbing cellular homeostasis. It reinforces the epithelial barrier by up-regulating hBD-1. Nevertheless, in pathogenic stress, R. nukuhivensis acts by preparing the immune system to...
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Ano: 2019 URL: https://archimer.ifremer.fr/doc/00659/77130/78499.pdf
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Tamanu oil and skin active properties: from traditional to modern cosmetic uses ArchiMer
Raharivelomanana, Phila; Ansel, Jean-luc; Lupo, Elise; Mijouin, Lily; Guillot, Samuel; Butaud, Jean-francois; Ho, Raimana; Lecellier, Gael; Pichon, Chantal.
Calophyllum inophyllum L. (Calophyllaceae), locally called "tamanu" in French Polynesia, is an evergreen pantropical tree growing mostly along the seashores. Its barks, leaves, and fruits are still used in traditional medicine. The oil expressed from the nuts has been also traditionally used. Tamanu oil is topically applied on skins as well as mucous membrane lesions. This oil is especially recommended to heal all kinds of skin ailments. Bioassays and different assessments of Tamanu oil revealed numerous biological activities (antioxidant, anti-inflammatory, antibacterial, wound healing...), so bringing scientific evidence of beneficial effects of this oil on human skin healing. Such biological properties may explain the use of tamanu oil as an active...
Tipo: Text Palavras-chave: Huile de tamanu; Calophyllum inophyllum; Cosmétopée; Néoflavonoïdes; Ingrédient cosmétique; Tamanu oil; Calophyllum inophyllum; Cosmetopoeia; Neoflavonoids; Cosmeceutical.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00660/77218/78679.pdf
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